Two simple, accurate, precise and specific spectrophotometric methods have been developed for simultaneous determination of Ceftriaxone Sodium (CFS) and Tazobactam Sodium (TAZO) in its combined Pharmaceutical formulation by using NaOH as a solvent. The first method is dual-wavelength method which involves estimation of CFS by absorbance difference at 248.48 nm and 265.78 nm, and TAZO by absorbance difference at 239.41 nm and 255.83 nm. The second method is first order derivative method in which absorption at 302.8 nm (zero cross point of TAZO) was used for quantification of CFS and 247.8 nm (zero cross point of CFS) for quantification of TAZO. Two methods follow Beer’s linearity in the range of 5-50μg/ml for CFS and 2.5-15 μg/ml for TAZO. The mean % recoveries were found to be in the range of 99.21 – 100.2 % and 99.52 – 100.26 % for CFS and TAZO respectively for dual-wavelength method and in the range of 98.84 – 99.69% and 100.08 – 100.73% CFS and TAZO respectively for first order derivative method. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of CFS and TAZO in their combined Pharmaceutical formulation.
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